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Pathologies such as exudative age-related macular decline (AMD) are characterized by choroidal neovascularization (CNV) — aberrant advance of new claret argosy from the choroid into the subretinal space.1,2 The laser-induced CNV (L-CNV) beastly archetypal of exudative AMD relies on laser photocoagulation to agitate Bruch’s membrane, appropriately aesthetic claret barge advance from the choriocapillaris into the subretinal space.3 This archetypal was originally developed in macaque4 and afterwards acclimatized for use in mice and rats. L-CNV has become the gold accustomed for preclinical testing of accustomed assay modalities acclimated for subretinal neovascularization.5–9 The archetypal has authentic invaluable for evaluating furnishings of biologic therapies on CNV bane progression in neovascular AMD research. 2,4,10,11 Cogent allowances of L-CNV accommodate its reproducibility and simplicity, which accommodate a aboveboard alignment for belief subretinal neovascularization.4

The L-CNV archetypal in non-human primates12 and in rodents8 undergoes three stages of development, artful the stages of development apparent in beastly CNV.12 The aboriginal date is an anarchic acknowledgment that initiates a advance phase.8 Soon afterwards laser photocoagulation, a gas balloon forms beneath the retina in the breadth of laser appliance (indicating disruption of Bruch’s membrane), and the retina becomes edematous and hazy.12 Three to 5 canicule afterwards laser photocoagulation (second stage), choroidal fibrovascular tissue proliferates.8,12 Seven to 10 canicule afterwards (

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